Robotics for single neuron recordings 

Robotics for single cell interfacing.jpg

These robotic tools that extend traditional single cell recording and manipulation techniques to high-throughput analyses of large numbers of cells simultaneously in intact tissue


B. D. Allen,  Caroline Moore-Kochlacs, J. G. Bernstein, J. Kinney, J. Scholvin, L. Seoane, C. Chronopoulos, C. Lamantia, S. B. Kodandaramaiah, M.Tegmark and E. S. Boyden, Automated in vivo patch clamp evaluation of extracellular multielectrode array spike recording capability, J Neurophysiology, 11 July 2018

S. B. Kodandaramaiah*, F. J. Flores* , G. L. Holst, A. C. Singer, X. Han , E. N. Brown, Edward S. Boyden^, C. R. Forest^ Multi-neuron intracellular recording in vivo via interacting autopatching robots, Elife 2018

* Equal Contribution, ^ Co-corresponding authors 

I. Kolb, G. Talei Franzesi, M. Wang, S. B. Kodandaramaiah, C. R. Forest, E. S. Boyden, and A. C. Singer, Evidence for long-timescale patterns of synaptic inputs in CA1 of awake behaving mice, J Neuroscience, 26 December 2017, 1519-17

H. J. Suk, I. Van Welie, S. B.  Kodandaramaiah, B. D.  Allen, C. R.  Forest, E. S.  Boyden, Closed-Loop Real-Time Imaging Enables Fully Automated Cell-Targeted Patch-Clamp Neural Recording In Vivo, Neuron 2017

Q. Wu, I. Kolb, B. Callahan, Z. Su, W. Stoy, S. B. Kodandaramaiah, R. Neve, H. Zeng, E.S. Boyden, C. R.  Forest, and Alexander Chubykin, Integration of autopatching with automated pipette and cell detection in vitro, Journal of Neurophysiology, 2016

S. B. Kodandaramaiah, I. R. Wickersham, G. B. Holst, A. C. Singer, G. Talei-Franzesi, C. R. Forest, E. S. Boyden, Assembly and operation of the autopatcher for automated intracellular neural recording in vivo, Nature Protocols, 2016

R. R. Harrison, I. Kolb, S. B. Kodandaramaiah, A. A. Chubykin, A. Yang, M. F. Bear, E. S. Boyden, C. R. Forest, Microchip amplifier for in vitro, in vivo, and automated whole-cell patch-clamp recording, J Neurophysiology, 2014

S. B. Kodandaramaiah, E.S. Boyden and C. R. Forest, E. S. Boyden, In Vivo Robotics: Towards the Automation of Neuroscience and Other Intact-System Biological Fields, Annals of New york Academy of Sciences, 2013

S.B. Kodandaramaiah, G. Talei Franzesi, B.Y. Chow, E.S. Boyden, C.R. Forest, Automated whole-cell patch clamp electrophysiology of neurons in vivo. Nature Methods2012